| distance | reference number | name | latitude | longitude |
| 24.9 | 2980 | Suffolk, Nansemond River, Virginia | 36.7333 | -76.5833 |
| 26.9 | 2825 | Smithfield, Pagan River, James River, Virginia | 36.9833 | -76.6333 |
| 27.7 | 1295 | Hollidays Point (bridge), Nansemond River, Virginia | 36.8333 | -76.55 |
| 28.0 | 388 | Burwell Bay, James River, Virginia | 37.05 | -76.6667 |
| 29.7 | 776 | Dillard Wharf, James River, Virginia | 37.2 | -76.8667 |
| 30.5 | 2708 | Scotland, Virginia | 37.1833 | -76.7833 |
| 30.6 | 2707 | Scotland, James River, Virginia | 37.185 | -76.7833 |
| 30.7 | 578 | Claremont, James River, Virginia | 37.2317 | -76.9483 |
| 31.4 | 1414 | Jamestown Island, James River, Virginia | 37.2 | -76.7833 |
| 31.4 | 3120 | Town Point, Nansemond River, Virginia | 36.8833 | -76.5 |
| 32.1 | 567 | Chuckatuck Creek entrance, James River, Virginia | 36.9167 | -76.5 |
| 33.0 | 1913 | Mulberry Point, James River, Virginia | 37.1333 | -76.6333 |
| 33.4 | 976 | Ferry Point (bridge), Chickahominy River, Virginia | 37.2667 | -76.8833 |
| 34.3 | 1290 | Hog Point, James River, Virginia | 37.2 | -76.6833 |
| 34.6 | 2976 | Sturgeon Point, James River, Virginia | 37.3 | -77 |
| 35.2 | 3361 | Windmill Point, James River, Virginia | 37.3 | -77.1 |
| 35.3 | 1807 | Menchville, James River, Virginia | 37.0833 | -76.5333 |
| 35.8 | 2246 | Pig Point, Nansemond River, Virginia | 36.9167 | -76.4333 |
| 36.0 | 1521 | Kingsmill, James River, Virginia | 37.22 | -76.6633 |
| 36.2 | 3345 | Willcox Wharf, Charles City, James River, Virginia | 37.3167 | -77.1 |
| 36.5 | 1350 | Huntington Park, James River, Virginia | 37.0167 | -76.4667 |
| 36.8 | 3311 | Westover, James River, Virginia | 37.3167 | -77.15 |
| 36.9 | 1996 | Newport News, James River, Virginia | 36.9667 | -76.4333 |
| 38.0 | 1900 | Mount Airy, Chickahominy River, Virginia | 37.35 | -76.9167 |
| 38.0 | 1442 | Jordan Point, James River, Virginia | 37.3167 | -77.2167 |